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two step primescript reverse transcription reagent kit  (TaKaRa)


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    Structured Review

    TaKaRa two step primescript reverse transcription reagent kit
    Two Step Primescript Reverse Transcription Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 88526 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/two+step+reverse+transcription+kit/PrimeScript+RT+Reagent+Kit/pmc12274960-136-1-10
    Average 99 stars, based on 88526 article reviews
    two step primescript reverse transcription reagent kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Expressional analysis of the silkworm storage protein 1 and identification of its interacting proteins.
    Article Snippet: Storage proteins are haemolymph-specific proteins in insects, mainly synthesized in the fat body, released into the haemolymph, and then selectively reabsorbed by the fat body before pupation.. These storage proteins play an important role in insect metamorphosis and egg development.. Some of these storage proteins are responsive to pathogen infection and can even suppress pathogen multiplication.

    Article Title: Expression and clinical significance of p73 in Wilms tumor in children
    Article Snippet: .. RNAiso Plus kit (Takara Biotechnology Co., Ltd., Dalian, China); Two-Step Reverse Transcription kit (Takara Bio, Inc., Otsu, Japan); SYBR-Green qPCR Master Mix kit (Thermo Fisher Scientific, Inc., Waltham, MA, USA); UV spectrophotometry instrument (Bio-Rad Laboratories, Inc., Hercules, CA, USA); RT-qPCR kit and StepOnePlus real-time PCR instrument (both from Thermor Fisher Scientific, Inc.). .. The primers of this study were designed by the Primer Premier 5.0 design software (Premier Biosoft, Inc., Palo Alto, CA, USA) and were generated by Tianjin Saier Biotechnology Co., Ltd. (Tianjin, China).

    Article Title: DGKA Mediates Resistance to PD-1 Blockade
    Article Snippet: Total RNA was extracted with TRIzol (Invitrogen) from OT-I T cells. .. RNA (500 ng) was used for reverse transcription to generate cDNA using the two-step reverse transcription kit (Takara). .. From 100 ng of cDNA template, quantitative real-time PCR (qRT-PCR) analysis for Dgka and Actb was performed by the CFX96 real-time PCR detection system (Bio-Rad) using their specific forward primers and reverse primers according to the manufacturer’s protocol (TransGen Biotech).

    Article Title: The Potential Role of As-sumo-1 in the Embryonic Diapause Process and Early Embryo Development of Artemia sinica
    Article Snippet: The 3′- and 5′- terminal fragments were spliced together using DNAman (Lynnon Biosoft) to yield the full length cDNA. .. mRNA samples were extracted from A. sinica at different time points (0 h, 5 h, 10 h, 15 h, 20 h and 40 h; 3, 5 d) and as templates for cDNA synthesis using a Two-step Reverse Transcription Kit (Takara). .. TaKaRa synthesized the primers for real-time PCR ( ).

    Article Title: HMGCS1 drives drug-resistance in acute myeloid leukemia through endoplasmic reticulum-UPR-mitochondria axis.
    Article Snippet: Total RNA was isolated from 1 × 106 cells using Trizol reagent (TaKaRa, No. 9108, Japan). .. Total cDNA was prepared using a two-step reverse transcription kit (TaKaRa, RR037A, Japan). .. Primers for real-time PCR were obtained from Integrated DNA Technologies.

    Article Title: Sorafenib-induced Aquaporin-3 Downregulation is Coupled With Proliferation Inhibition and Increased Apoptosis in Hepatocellular Carcinoma Cells
    Article Snippet: RNA extraction, reverse transcription, and qPCR Total RNA was extracted from cells using an RNAIsoPlus assay kit (Takara, Dalian, China) according to the manufacturer’s instructions. .. After quanti cation, RNA was transcribed into cDNA using a two-step reverse transcription kit (Takara, Dalian, China). .. Subsequently, qPCR was used to detect target gene expression levels using the SYBR-Green qPCR master mix (Takara, Dalian, China).

    Article Title: Parabacteroides distasonis uses dietary inulin to suppress NASH via its metabolite pentadecanoic acid
    Article Snippet: The integrity and purity of the RNA were determined using a nanodrop spectrophotometer (Thermo Fisher Scientific) and electrophoresis. .. Complementary DNA was obtained via a two-step reverse transcription kit (TaKaRa). .. RNA-seq was performed by Novogene.

    Article Title: Molecular characterization and expression analysis of protein enhancer of sevenless 2B from Artemia sinica at early embryonic development and during immune response to bacterial stimulation.
    Article Snippet: Protein enhancer of sevenless 2B, E(sev)2B, is a key adapter protein in the Ras/MAPK signaling pathway which has been reported to be involved in innate immunity.. In this study, the gene that encodes AsE(sev)2B was isolated from A. sinica.. It was found to contain a 636 bp open reading frame encoding 211 amino acids with a calculated molecular mass of 24.357 kDa and a predicted isoelectric point of 5.39.

    SYBR Green Assay:

    Article Title: Expression and clinical significance of p73 in Wilms tumor in children
    Article Snippet: .. RNAiso Plus kit (Takara Biotechnology Co., Ltd., Dalian, China); Two-Step Reverse Transcription kit (Takara Bio, Inc., Otsu, Japan); SYBR-Green qPCR Master Mix kit (Thermo Fisher Scientific, Inc., Waltham, MA, USA); UV spectrophotometry instrument (Bio-Rad Laboratories, Inc., Hercules, CA, USA); RT-qPCR kit and StepOnePlus real-time PCR instrument (both from Thermor Fisher Scientific, Inc.). .. The primers of this study were designed by the Primer Premier 5.0 design software (Premier Biosoft, Inc., Palo Alto, CA, USA) and were generated by Tianjin Saier Biotechnology Co., Ltd. (Tianjin, China).

    Real-time Polymerase Chain Reaction:

    Article Title: Expression and clinical significance of p73 in Wilms tumor in children
    Article Snippet: .. RNAiso Plus kit (Takara Biotechnology Co., Ltd., Dalian, China); Two-Step Reverse Transcription kit (Takara Bio, Inc., Otsu, Japan); SYBR-Green qPCR Master Mix kit (Thermo Fisher Scientific, Inc., Waltham, MA, USA); UV spectrophotometry instrument (Bio-Rad Laboratories, Inc., Hercules, CA, USA); RT-qPCR kit and StepOnePlus real-time PCR instrument (both from Thermor Fisher Scientific, Inc.). .. The primers of this study were designed by the Primer Premier 5.0 design software (Premier Biosoft, Inc., Palo Alto, CA, USA) and were generated by Tianjin Saier Biotechnology Co., Ltd. (Tianjin, China).

    Spectrophotometry:

    Article Title: Expression and clinical significance of p73 in Wilms tumor in children
    Article Snippet: .. RNAiso Plus kit (Takara Biotechnology Co., Ltd., Dalian, China); Two-Step Reverse Transcription kit (Takara Bio, Inc., Otsu, Japan); SYBR-Green qPCR Master Mix kit (Thermo Fisher Scientific, Inc., Waltham, MA, USA); UV spectrophotometry instrument (Bio-Rad Laboratories, Inc., Hercules, CA, USA); RT-qPCR kit and StepOnePlus real-time PCR instrument (both from Thermor Fisher Scientific, Inc.). .. The primers of this study were designed by the Primer Premier 5.0 design software (Premier Biosoft, Inc., Palo Alto, CA, USA) and were generated by Tianjin Saier Biotechnology Co., Ltd. (Tianjin, China).

    Quantitative RT-PCR:

    Article Title: Expression and clinical significance of p73 in Wilms tumor in children
    Article Snippet: .. RNAiso Plus kit (Takara Biotechnology Co., Ltd., Dalian, China); Two-Step Reverse Transcription kit (Takara Bio, Inc., Otsu, Japan); SYBR-Green qPCR Master Mix kit (Thermo Fisher Scientific, Inc., Waltham, MA, USA); UV spectrophotometry instrument (Bio-Rad Laboratories, Inc., Hercules, CA, USA); RT-qPCR kit and StepOnePlus real-time PCR instrument (both from Thermor Fisher Scientific, Inc.). .. The primers of this study were designed by the Primer Premier 5.0 design software (Premier Biosoft, Inc., Palo Alto, CA, USA) and were generated by Tianjin Saier Biotechnology Co., Ltd. (Tianjin, China).

    cDNA Synthesis:

    Article Title: The Potential Role of As-sumo-1 in the Embryonic Diapause Process and Early Embryo Development of Artemia sinica
    Article Snippet: The 3′- and 5′- terminal fragments were spliced together using DNAman (Lynnon Biosoft) to yield the full length cDNA. .. mRNA samples were extracted from A. sinica at different time points (0 h, 5 h, 10 h, 15 h, 20 h and 40 h; 3, 5 d) and as templates for cDNA synthesis using a Two-step Reverse Transcription Kit (Takara). .. TaKaRa synthesized the primers for real-time PCR ( ).

    Article Title: Molecular characterization and expression analysis of protein enhancer of sevenless 2B from Artemia sinica at early embryonic development and during immune response to bacterial stimulation.
    Article Snippet: Protein enhancer of sevenless 2B, E(sev)2B, is a key adapter protein in the Ras/MAPK signaling pathway which has been reported to be involved in innate immunity.. In this study, the gene that encodes AsE(sev)2B was isolated from A. sinica.. It was found to contain a 636 bp open reading frame encoding 211 amino acids with a calculated molecular mass of 24.357 kDa and a predicted isoelectric point of 5.39.

    Concentration Assay:

    Article Title: Molecular characterization and expression analysis of protein enhancer of sevenless 2B from Artemia sinica at early embryonic development and during immune response to bacterial stimulation.
    Article Snippet: Protein enhancer of sevenless 2B, E(sev)2B, is a key adapter protein in the Ras/MAPK signaling pathway which has been reported to be involved in innate immunity.. In this study, the gene that encodes AsE(sev)2B was isolated from A. sinica.. It was found to contain a 636 bp open reading frame encoding 211 amino acids with a calculated molecular mass of 24.357 kDa and a predicted isoelectric point of 5.39.

    Control:

    Article Title: Molecular characterization and expression analysis of protein enhancer of sevenless 2B from Artemia sinica at early embryonic development and during immune response to bacterial stimulation.
    Article Snippet: Protein enhancer of sevenless 2B, E(sev)2B, is a key adapter protein in the Ras/MAPK signaling pathway which has been reported to be involved in innate immunity.. In this study, the gene that encodes AsE(sev)2B was isolated from A. sinica.. It was found to contain a 636 bp open reading frame encoding 211 amino acids with a calculated molecular mass of 24.357 kDa and a predicted isoelectric point of 5.39.

    Targeted Gene Expression:

    Article Title: Molecular characterization and expression analysis of protein enhancer of sevenless 2B from Artemia sinica at early embryonic development and during immune response to bacterial stimulation.
    Article Snippet: Protein enhancer of sevenless 2B, E(sev)2B, is a key adapter protein in the Ras/MAPK signaling pathway which has been reported to be involved in innate immunity.. In this study, the gene that encodes AsE(sev)2B was isolated from A. sinica.. It was found to contain a 636 bp open reading frame encoding 211 amino acids with a calculated molecular mass of 24.357 kDa and a predicted isoelectric point of 5.39.



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